Tag: polyclonal ab

Viral cDNA from capsid-like structures in the nucleus

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HIV-1 uncoating by release of viral cDNA from capsid-like structures in the nucleus of infected cells

HIV-1 replication commences inside the cone-shaped viral capsid, but timing, localization and mechanism of uncoating are under debate. We adapted a strategy to visualize individual reverse-transcribed HIV-1 cDNA molecules and their association with viral and cellular proteins using fluorescence and correlative-light-and-electron-microscopy (CLEM). We specifically detected HIV-1 cDNA inside nuclei, but not in the cytoplasm. Nuclear cDNA initially co-localized with a fluorescent integrase fusion (IN-FP) and the viral CA (capsid) protein, but cDNA-punctae separated from IN-FP/CA over time.

This phenotype was conserved in primary HIV-1 target cells, with nuclear HIV-1 complexes exhibiting strong CA-signals in all cell types. CLEM revealed cone-shaped HIV-1 capsid-like structures and apparently broken capsid-remnants at the position of IN-FP signals and elongated chromatin-like structures in the position of viral cDNA punctae lacking IN-FP. Our data argue for nuclear uncoating by physical disruption rather than cooperative disassembly of the CA-lattice, followed by physical separation from the pre-integration complex.

 

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney
C8235142-PP 10 reactions x2
EUR 408.1

Normal kidney and normal adjacent kidney tissue array
KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Kidney cancer tissue array with normal Kidney tissue
KD2082a each
EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Kidney Tissue Slide (Normal)
10-401-10um 10 um
EUR 241.8

Kidney Tissue Slide (Normal)
10-401-4um 4 um
EUR 216.6

Normal kidney tissue array
KD803 each
EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array
KDN242 each
EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Kidney Tissue Slide (Normal)
MBS154338-10um 10um
EUR 210

Kidney Tissue Slide (Normal)
MBS154338-4um 4um
EUR 195

Kidney Tissue Slide (Normal)
MBS154338-5x10um 5x10um
EUR 920

Tissue cDNA, First Strand, Human Adult Normal, Kidney, BioGenomics
MBS652261-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Human Adult Normal, Kidney, BioGenomics
MBS652261-5x40Tests 5x40Tests
EUR 2835

Tissue cDNA, First Strand, Mouse Adult Normal, Kidney, BioGenomics
MBS652496-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Mouse Adult Normal, Kidney, BioGenomics
MBS652496-5x40Tests 5x40Tests
EUR 2835

Kidney Tissue Lysate (Normal)
1706-04 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-05 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-02 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-03 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Tissue cDNA, First Strand, Canine Adult Normal, Kidney, BioGenomics
MBS652439-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Canine Adult Normal, Kidney, BioGenomics
MBS652439-5x40Tests 5x40Tests
EUR 2835

Rat Tissue Lysate Kidney Medulla Normal
GWB-F5DEBF 0.15 mg Ask for price

Kidney cancer tissue array with adjacent normal kidney tissue
BC07015b each
EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Genomic DNA - Mouse Normal Tissue: Kidney
D1334142 100 ug
EUR 241.5

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS652552-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS652552-5x40Tests 5x40Tests
EUR 2835

Tissue Lysate (Normal) Human Kidney
GWB-FEAC28 0.15 mg Ask for price

Kidney cancer tissue array with matched adjacent normal kidney tissue
KD901a each
EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Kidney, BioGenomics
MBS652401-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Kidney, BioGenomics
MBS652401-5x40Tests 5x40Tests
EUR 2835

Kidney tumour with normal tissue array
KD2001 each
EUR 474
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80263 1 x 100 ml
EUR 132.3

Kidney cancer tissue array with normal tissues
KD2083 each
EUR 474
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores

Kidney cancer tissue array with normal tissues
KD721 each
EUR 168
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores

Tissue cDNA (Matched Pairs), Human Primary Tumor and Normal, Kidney, BioGenomics
MBS652533-2Panels 2Panels
EUR 805

Tissue cDNA (Matched Pairs), Human Primary Tumor and Normal, Kidney, BioGenomics
MBS652533-5x2Panels 5x2Panels
EUR 3400

Mouse Normal Tissue: Kidney Whole Cell Lysate
MNK-100 1 mg
EUR 628.8

Mouse Normal Tissue: Kidney Whole Cell Lysate
MNK-50 50 ug
EUR 196.8

Kidney tumor tissue array with normal tissue as control
KD961 each
EUR 258
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Kidney cancer tissue array with normal tissue as control
KD962 each
EUR 258
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Tissue, Total RNA, Rat Adult Normal, Kidney, BioGenomics
MBS638687-005mg 0.05mg
EUR 435

Tissue, Total RNA, Rat Adult Normal, Kidney, BioGenomics
MBS638687-5x005mg 5x0.05mg
EUR 1735

Adjacent normal kidney tissue and cancer tissue array
BN07011b each
EUR 203
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011

Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue
KD701a each
EUR 234
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701

FFPE Total RNA - Human Adult Normal Tissue: Kidney
R2234142 1 ug
EUR 744

Tissue, Genomic DNA, Rat Adult Normal, Kidney, BioGenomics
MBS654611-01mg 0.1mg
EUR 575

Tissue, Genomic DNA, Rat Adult Normal, Kidney, BioGenomics
MBS654611-5x01mg 5x0.1mg
EUR 2440

Kidney cancer tissue array with matched adjacent normal tissue
KD321a each
EUR 198
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321

Kidney cancer with matched adjacent normal kidney tissue array
KD603 each
EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue
Kid-060Sur01 each
EUR 954
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores

Human Adult Kidney (Normal) Whole tissue lysate
HAL-1305 1 mg
EUR 628.8

Monkey (Cyno.) Normal Kidney Whole tissue lysate
MCL-1190 1 mg
EUR 628.8

Tissue, Total Protein, Mouse Adult Normal, Kidney
MBS657420-1mg 1mg
EUR 565

Tissue, Total Protein, Mouse Adult Normal, Kidney
MBS657420-5x1mg 5x1mg
EUR 2315

Tissue, Total Protein, Human Adult Normal, Kidney
MBS657589-1mg 1mg
EUR 565

Tissue, Total Protein, Human Adult Normal, Kidney
MBS657589-5x1mg 5x1mg
EUR 2315

Tissue, Section, Human Adult Normal, Kidney (Frozen)
MBS640178-5Sections 5Sections
EUR 635

Tissue, Section, Human Adult Normal, Kidney (Frozen)
MBS640178-5x5Sections 5x5Sections
EUR 2640

Monkey (Rhesus) Normal Kidney Whole tissue lysate
MRL-1301 1 mg
EUR 628.8

Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Kidney
MBS640973-1Set 1Set
EUR 650

Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Kidney
MBS640973-5x1Set 5x1Set
EUR 2970

Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues
Z7020053 5 slides
EUR 1125.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Tissue, Section, Human Adult Normal, Kidney (Paraffin)
MBS640009-5Sections 5Sections
EUR 460

Tissue, Section, Human Adult Normal, Kidney (Paraffin)
MBS640009-5x5Sections 5x5Sections
EUR 1925

Tissue, Membrane Protein, Human Adult Normal, Kidney
MBS639828-01mg 0.1mg
EUR 570

Tissue, Membrane Protein, Human Adult Normal, Kidney
MBS639828-5x01mg 5x0.1mg
EUR 2345

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80075 1 x 100 ml
EUR 160.65

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80171 1 x 100 ml
EUR 160.65

Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts
9-80267 1 x 100 ml
EUR 208.85

Multiple Kidney cancer tissue array with normal tissue control from autopsy
KD483 each
EUR 168
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data

Rat Normal Kidney tissue (single section per slide) (5 slides/pack)
RaFPT007 5 slides
EUR 150

Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack
TS-R5405 -
EUR 145

Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney
D8235142-PP-10 2x10 ug
EUR 334.6

cDNA - Rat Normal Tissue: Lung
C1434152 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Brain
C1434035 40 reactions
EUR 317.45

cDNA - Rat Normal Tissue: Colon
C1434090 40 reactions
EUR 467.25

cDNA - Rat Normal Tissue: Heart
C1434122 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Liver
C1434149 40 reactions
EUR 311.5

Tissue, Total RNA, Mouse, Adult Normal, Kidney, BioGenomics
MBS638539-005mg 0.05mg
EUR 435

Tissue, Total RNA, Mouse, Adult Normal, Kidney, BioGenomics
MBS638539-5x005mg 5x0.05mg
EUR 1735

Tissue, Total RNA, Human Adult Normal, Kidney, BioGenomics
MBS638594-005mg 0.05mg
EUR 495

Tissue, Total RNA, Human Adult Normal, Kidney, BioGenomics
MBS638594-5x005mg 5x0.05mg
EUR 1995

cDNA - Rat Normal Tissue: Rectum
C1434206 40 reactions
EUR 467.25

cDNA - Rat Normal Tissue: Spleen
C1434246 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Testis
C1434260 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Adipose
C1434003 40 reactions
EUR 467.25

cDNA - Rat Normal Tissue: Bladder
C1434010 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Stomach
C1434248 40 reactions
EUR 311.5

High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue
KD208 each
EUR 546
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

cDNA - Rat Normal Tissue: Placenta
C1434200 40 reactions
EUR 467.25

Tissue, Genomic DNA, Mouse Adult Normal, Kidney, BioGenomics
MBS654413-01mg 0.1mg
EUR 575

Tissue, Genomic DNA, Mouse Adult Normal, Kidney, BioGenomics
MBS654413-5x01mg 5x0.1mg
EUR 2440

Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor
D1234142 100 ug
EUR 241.5

Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes
9-80172 1 x 100 ml
EUR 160.65

Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells
9-80262 1 x 100 ml
EUR 132.3

Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue
KD242 each
EUR 48
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array
KD241 each
EUR 48
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array
KD241a each
EUR 66
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241

Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells
9-80266 1 x 100 ml
EUR 132.3

Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells
9-80269 1 x 100 ml
EUR 132.3

Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS638547-005mg 0.05mg
EUR 485

Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS638547-5x005mg 5x0.05mg
EUR 1960

Tissue Set, BioAssay, DNA, RNA, Protein, Human Adult Normal, Kidney
MBS639416-1Kit 1Kit
EUR 730

Tissue Set, BioAssay, DNA, RNA, Protein, Human Adult Normal, Kidney
MBS639416-5x1Kit 5x1Kit
EUR 3075

Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Kidney
MBS640995-1Set 1Set
EUR 650

Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Kidney
MBS640995-5x1Set 5x1Set
EUR 2970

Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes
9-80079 1 x 100 ml
EUR 160.65

cDNA - Rat Normal Tissue: Skeletal Muscle
C1434171 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Small Intestine
C1434226 40 reactions
EUR 467.25

Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts
9-80077 1 x 100 ml
EUR 160.65

Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts
9-80174 1 x 100 ml
EUR 208.85

 

Cloning symbiosis-related cDNAs from eucalypt ectomycorrhiza by PCR-assisted differential screening

 

As part of a project to identify symbiosis-related genes, we report here a simple differential screening procedure for isolating up- and down-regulated fungal transcripts from a cDNA library of the developing Eucalyptus globulus-Pisolithus tinctorius mycorrhiza. cDNA inserts of randomly selected λZAP plaques were amplified by PCR and separated by agarose gel electrophoresis.

The PCR-amplified cDNA samples were then screened by Southern blotting, using radiolabelled-cDNA probes of high specific activity. We have applied this method to fungal transcripts that are differentially expressed in ectomycorrhizas during the early stages of development. We estimate that about 50 % of the fungal mRNA population is regulated by development of the symbiosis; several up- and down-regulated cDNAs have been isolated for further analysis.

Characterization of an Aux/IAA cDNA upregulated in Pinus pinaster roots in response to colonization by the ectomycorrhizal fungus Hebeloma cylindrosporum

 

  • In an attempt to determine whether fungal auxin affects host plant gene expression during mycorrhizal formation, an auxin upregulated cDNA, Pp-iaa88, was isolated by differential screening of a cDNA library made from auxin-treated Pinus pinaster roots. • Pp-iaa88 codes for a polypeptide that shares extensive homology to auxin-inducible Aux/IAA proteins, which are supposed to act as transcription factors. Cycloheximide did not inhibit auxin-induced mRNA accumulation, indicating that Pp-iaa88 upregulation is a primary (direct) auxin response.
  • The level of Pp-iaa88 transcripts in roots increased following inoculation with either an indoleacetic acid-overproducing mutant or a wild-type strain of the ectomycorrhizal fungus Hebeloma cylindrosporum. With both strains, mRNA accumulation was detectable as soon as fungal hyphae reached the root and it increased during differentiation of symbiotic structures. The kinetics of Pp-iaa88 transcript accumulation was closely connected with the dynamics of symbiosis establishment and was more rapid with the mutant than with the wild-type strain.
  • As a putative transcription factor expressed at the very early stages of symbiosis establishment, Pp-iaa88 could play a key role in mycorrhizal formation.

 

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Complete Genome Sequencing and Infectious cDNA Clone Construction of Soybean Mosaic Virus Isolated from Shanxi

 

Soybean mosaic virus (SMV) is the predominant viral pathogen that affects the yield and quality of soybean. The natural host range for SMV is very narrow, and generally limited to Leguminosae. However, we found that SMV can naturally infect Pinellia ternata and Atractylodes macrocephala. In order to clarify the molecular mechanisms underlying the crossfamily infection of SMV, we used double-stranded RNA extraction, rapid amplification of cDNA ends polymerase chain reaction and Gibson assembly techniques to carry out SMV full-length genome amplification from susceptible soybeans and constructed an infectious cDNA clone for SMV.

The genome of the SMV Shanxi isolate (SMV-SX) consists of 9,587 nt and encodes a polyprotein consisting of 3,067 aa. SMV-SX and SMV-XFQ008 had the highest nucleotide and amino acid sequence identities of 97.03% and 98.50%, respectively. A phylogenetic tree indicated that SMV-SX and SMV-XFQ018 were clustered together, sharing the closest relationship. We then constructed a pSMV-SX infectious cDNA clone by Gibson assembly technology and used this clone to inoculate soybean and Ailanthus altissima; the symptoms of these hosts were similar to those caused by the virus isolated from natural infected plant tissue.

This method of construction not only makes up for the time-consuming and laborious defect of traditional methods used to construct infectious cDNA clones, but also avoids the toxicity of the Potyvirus special sequence to Escherichia coli, thus providing a useful cloning strategy for the construction of infectious cDNA clones for other viruses and laying down a foundation for the further investigation of SMV cross-family infection mechanisms.

 

Effect of thermospermine on expression profiling of different gene using massive analysis of cDNA ends (MACE) and vascular maintenance in Arabidopsis

 

Arabidopsis thaliana polyamine oxidase 5 gene (AtPAO5) functions as a thermospermine (T-Spm) oxidase. Aerial growth of its knock-out mutant (Atpao5-2) was significantly repressed by low dose(s) of T-Spm but not by other polyamines. To figure out the underlying mechanism, massive analysis of 3′-cDNA ends was performed. Low dose of T-Spm treatment modulates more than two fold expression 1,398 genes in WT compared to 3186 genes in Atpao5-2. Cell wall, lipid and secondary metabolisms were dramatically affected in low dose T-Spm-treated Atpao5-2, in comparison to other pathways such as TCA cycle-, amino acid- metabolisms and photosynthesis.

The cell wall pectin metabolism, cell wall proteins and degradation process were highly modulated. Intriguingly Fe-deficiency responsive genes and drought stress-induced genes were also up-regulated, suggesting the importance of thermospermi’ne flux on regulation of gene network. Histological observation showed that the vascular system of the joint part between stem and leaves was structurally dissociated, indicating its involvement in vascular maintenance. Endogenous increase in T-Spm and reduction in H2O2 contents were found in mutant grown in T-Spm containing media. The results indicate that T-Spm homeostasis by a fine tuned balance of its synthesis and catabolism is important for maintaining gene regulation network and the vascular system in plants.

 

Molecular characterization of a novel p38 MAPK cDNA from Cyclina sinensis and its potential immune-related function under the threat of Vibrio anguillarum

 

The p38 mitogen-activated protein kinase (MAPK) is one important member of MAPK family and reported to serve a predominant function in regulating innate immunity after the occurrence of certain infection. In the present study, one novel p38 MAPK gene was acquired from Cyclina sinensis based on the RNA-seq analysis and designated as Csp38 MAPK. This novel gene contained a full length of 1781 bp, 1104 bp of which was deemed as open reading frames and gave rise to a peptide of 367 amino acids with a predicted molecular weight of 42.31 KDa. A conserved serine/threonine protein kinase (S_Tkc) region along with a Thr-Gly-Tyr motif was discovered in the deduced sequence.

According to the phylogenetic analysis, there was a close relationship between this kinase and Meretrix petechialis p38 MAPK. As for the expression pattern, this newly-identified Csp38 MAPK was ubiquitously distributed in several tissues throughout the body but with varied abundance. After the challenge of Vibrio anguillarum, both the transcription and phosphorylation level of Csp38 MAPK in hemolymph were coordinately altered with a time-dependent manner. Besides, with the application of double strand RNA homologous to myeloid differentiation factor 88 (MyD88) of C. sinensis, the activation of Csp38 MAPK was found to obviously decrease in hemolymph after the pathogen stimulation. Hence, our experimental data presented evidence for the potential involvement of p38 MAPK in response to bacterial invaders in C. sinensis, possibly facilitating our understanding for pathogen-induced innate immunity in clams.

 

Cat Uterus Genomic DNA
FG-411 0.1mg
EUR 210

Cat Uterus Total Protein
FT-411 1mg
EUR 176

cDNA - Human Tumor Tissue: Uterus
C1235274 40 reactions
EUR 467.25

Rat Uterus, non-pregnant cDNA
RD-411 30 reactions
EUR 243

Mouse CD1 Uterus, non-pregnant cDNA
MD-411 30 reactions
EUR 243

Mouse BLC Uterus, non-pregnant cDNA
MD-411-BLC 30 reactions
EUR 280

Mouse C57 Uterus, non-pregnant cDNA
MD-411-C57 30 reactions
EUR 280

cDNA - Human Adult Normal Tissue: Uterus
C1234274 40 reactions
EUR 311.5

cDNA - Monkey (Rhesus) Normal Tissue: Uterus
C1534274 40 reactions
EUR 531.3

cDNA - Monkey (Cynomolgus) Normal Tissue: Uterus
C1534274-Cy 40 reactions
EUR 531.3

cDNA - Human Adult Normal Tissue: Uterus: Cervix
C1234275 40 reactions
EUR 311.5

cDNA - Human Adult Normal Tissue: Uterus: Corpus
C1234276-10 10 reactions
EUR 173.95

cDNA - Human Adult Normal Tissue: Uterus: Fundus
C1234278-10 10 reactions
EUR 173.95

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Cervix
C1534275 40 reactions
EUR 531.3

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Corpus
C1534276 40 reactions
EUR 531.3

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Fundus
C1534278 40 reactions
EUR 531.3

Rat Uterus, non-pregnant cDNA-Random Primer
RD-411-RH 30 reactions
EUR 243

cDNA - Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix
C1534275-Cy 40 reactions
EUR 531.3

Rat WS Uterus, non-pregnant cDNA-Oligo-dT
RD-411-WS 30 reactions
EUR 243

Mouse C57 Uterus, non-pregnant cDNA-Random Primer
MD-411-C57-RH 30 reactions
EUR 280

Mouse CD1 Uterus, non-pregnant cDNA-Random Primer
MD-411-HR 30 reactions
EUR 243

Tissue cDNA, First Strand, Human Tumor, Uterus, BioGenomics
MBS651979-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Human Tumor, Uterus, BioGenomics
MBS651979-5x40Tests 5x40Tests
EUR 3600

Tissue cDNA, First Strand, Human Adult Normal, Uterus, BioGenomics
MBS651999-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Human Adult Normal, Uterus, BioGenomics
MBS651999-5x40Tests 5x40Tests
EUR 2835

Tissue cDNA, First Strand, Human Adult Normal, Uterus, Cervix, BioGenomics
MBS651890-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Human Adult Normal, Uterus, Cervix, BioGenomics
MBS651890-5x40Tests 5x40Tests
EUR 2835

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Uterus, BioGenomics
MBS652015-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Uterus, BioGenomics
MBS652015-5x40Tests 5x40Tests
EUR 3600

Tissue cDNA, First Strand, Human Adult Normal, Uterus, Corpus, BioGenomics
MBS652435-10Tests 10Tests
EUR 540

Tissue cDNA, First Strand, Human Adult Normal, Uterus, Corpus, BioGenomics
MBS652435-5x10Tests 5x10Tests
EUR 2215

Tissue cDNA, First Strand, Human Adult Normal, Uterus, Fundus, BioGenomics
MBS652490-10Tests 10Tests
EUR 540

Tissue cDNA, First Strand, Human Adult Normal, Uterus, Fundus, BioGenomics
MBS652490-5x10Tests 5x10Tests
EUR 2215

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Uterus, BioGenomics
MBS652498-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Uterus, BioGenomics
MBS652498-5x40Tests 5x40Tests
EUR 3600

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Uterus, Fundus, BioGenomics
MBS652111-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Uterus, Fundus, BioGenomics
MBS652111-5x40Tests 5x40Tests
EUR 3600

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Uterus, Corpus, BioGenomics
MBS652277-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Uterus, Corpus, BioGenomics
MBS652277-5x40Tests 5x40Tests
EUR 3600

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Uterus, Cervix, BioGenomics
MBS652482-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Uterus, Cervix, BioGenomics
MBS652482-5x40Tests 5x40Tests
EUR 3600

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Uterus, Cervis, BioGenomics
MBS651910-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Uterus, Cervis, BioGenomics
MBS651910-5x40Tests 5x40Tests
EUR 3600

Uterus, Bovine
MBS639365-5Each 5Each
EUR 395

Uterus, Bovine
MBS639365-5x5Each 5x5Each
EUR 1550

Uterus, Rabbit
MBS639336-25Each 25Each
EUR 395

Uterus, Rabbit
MBS639336-5x25Each 5x25Each
EUR 1550

Uterus Lysate
MBS151639-01mg 0.1mg
EUR 200

Uterus Lysate
MBS151639-5x01mg 5x0.1mg
EUR 880

Dog Uterus RNA*
DR-411 0.05mg
EUR 195

BOVINE, UTERUS, FRESH
8600861 1EA
EUR 67.09

Uterus Tumor Lysate
MBS151717-01mg 0.1mg
EUR 260

Uterus Tumor Lysate
MBS151717-5x01mg 5x0.1mg
EUR 1140

Fetal Uterus Lysate
XBL-10431 0.1 mg
EUR 632.4
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

PORCINE, UTERUS, FRESH
8604961 1EA
EUR 65.63

BOVINE, UTERUS, FROZEN
8620861 1EA
EUR 67.09

PORCINE, UTERUS, FROZEN
8624961 1EA
EUR 65.63

Rat Uterus Fibroblasts
ABC-TC4237 1 vial Ask for price
Description: Rat uterus fibroblasts, 6-week Wistar rat

OORA00351-10U - UTERUS
OORA00351-10U 1Each
EUR 105

OORA00351-1EA - UTERUS
OORA00351-1EA 1Each
EUR 99

OORA00493-10U - UTERUS
OORA00493-10U 1Each
EUR 55

OORA00493-1EA - UTERUS
OORA00493-1EA 1Each
EUR 59

Uterus Membrane Lysate
XBL-11023 0.1 mg
EUR 619.8
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Tissue, Total RNA, Human Adult Normal, Uterus, Cervix of uterus, BioGenomics
MBS638538-005mg 0.05mg
EUR 495

Tissue, Total RNA, Human Adult Normal, Uterus, Cervix of uterus, BioGenomics
MBS638538-5x005mg 5x0.05mg
EUR 1995

Tissue, Total RNA, Human Adult Normal, Uterus, Corpus of Uterus, BioGenomics
MBS638682-001mg 0.01mg
EUR 485

Tissue, Total RNA, Human Adult Normal, Uterus, Corpus of Uterus, BioGenomics
MBS638682-5x001mg 5x0.01mg
EUR 1960

Pig Uterus Total RNA
PR-411 0.1mg
EUR 235

Dog Uterus Genomic DNA
DG-411 0.1mg
EUR 210

Pig Uterus Genomic DNA
PG-411 0.1mg
EUR 177

Sheep Uterus Total RNA
SR-411 0.1mg
EUR 160

Rat Uterus Genomic DNA
RG-411 0.1mg
EUR 177

Bovine Uterus Total RNA
BR-411 0.1mg
EUR 160

Rabbit Uterus Total RNA
TR-411 0.1mg
EUR 160

Hamster Uterus Total RNA*
AR-411 0.05mg
EUR 160

Dog Uterus Total Protein
DT-411 1mg
EUR 176

Human Uterus Genomic DNA
HG-411 0.05mg
EUR 210

Pig Uterus Total Protein
PT-411 1mg
EUR 153

MiniPig Uterus Total RNA
NR-411 0.1mg
EUR 231

Mouse Uterus Genomic DNA
MG-411 0.1mg
EUR 177

Sheep Uterus Genomic DNA
SG-411 0.1mg
EUR 177

Bovine Uterus Genomic DNA
BG-411 0.1mg
EUR 177

Equine Uterus Genomic DNA
GE-411 0.1mg
EUR 210

Human Uterus Tumor lysate
HTL-1384 1 mg
EUR 927.6

OORA00552-1U - Rat UTERUS
OORA00552-1U 1Each
EUR 60

Rabbit Uterus Genomic DNA
TG-411 0.1mg
EUR 177

Uterus tumor tissue array
UT802a each
EUR 306
Description: Uterus tumor tissue array, including stromal and leiomyo sarcoma, endometrioid adenocarcinoma, chorionepithelioma, hydatidiform mole, clear cell carcinoma, carcinsarcoma, sarcomatoid carcinoma, with pathology grade, TNM/Stage (AJCC 8th edition), 80 cases/80 cores (cores size 1.5mm), replacing UT802

Uterus Tissue Slide (Normal)
11-401-10um 10 um
EUR 241.8

Uterus Tissue Slide (Normal)
11-401-4um 4 um
EUR 216.6

Uterus Tissue Slide (Benign)
11-402-10um 10 um
EUR 241.8
Categories: cellculture, control, immune Tags: Tags: , , , , , , , , ,